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Bioethanol and biohydrogen have emerged as significant renewable options However, these bioprocess routes have presented various challenges, which constantly impede commercialization. Pretreatment, saccharification (hydrolysis), fermentation, and purification as shown in figures 6 and 7 The production of cellulase cocktails has been widely explored, however, there are still some main challenges that enzymes need to overcome in order to develop a sustainable production of. Production system figure 1 presents the block diagram of the ethanol production process adopted in this study Scheme of the ethanol production. Schematic diagram of ethanol production process from biosyngas (thermochemical conversion) Bioethanol is recognized as an important renewable and sustainable transportation fuel. In the first method , according to fig 2, bioethanol is produced from dry microalgae In the second method , according to fig 3, wet microalgae are used to produce bioethanol. 3 shows that the unit costs of ethanol production depend on raw material, capacity and investment cost Process flow diagram of the bioethanol production process from spent sugar beet pulp, consisting of pretreatment (acid hydrolysis) and saccharification (enzyme hydrolysis) stages. Bioethanol production from cereal grains comprises the following main stages Milling, starch hydrolysis, yeast fermentation, distillation (to ~95% ethanol) and water removal from ethanol (to 99.9% or absolute ethanol).